AFFINITY CHROMATOGRAPHY ISOLATION AND CHARACTERIZATION OF SOLUBLE cGMP-BINDING PROTEINS FROM AVENA SATIVA L. SEEDLINGS
نویسندگان
چکیده
In attempt to elucidate the early molecular events associated with the biological action of cyclic guanosine 3/,5/monophosphate (cGMP) its binding to the structural components of Avena sativa L. plant cell has been studied. cGMP was shown to be bound specifically to proteins located predominantly in the soluble cytosolic fraction. cGMP-agarose affinity purification procedure followed by SDS-PAGE has revealed about ten specific cGMP-binding proteins in the cytosol. The nature of the purified proteins has been discussed..
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